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00:00 and your reporting? Yeah. Okay folks, um let's uh you're

00:23 your hair. I um we don't a whole lot to finish up.

00:29 make it out of here early. uh the usual stuff. So just

00:38 , you know, quiz. That's gonna be more and more

00:44 I think there's 25 questions on Um What else? Smart work.

00:52 have three. You have three. open all that stuff is available.

00:56 did that yesterday. So you have that, all the stuff you need

00:59 unit three. We'll start that next . Obviously the stuff we're talking about

01:04 week is not on the exam, ? Unit three is stuff is exam

01:09 . Uh So of course a week tomorrow, I guess exam too.

01:16 , so um let's see the uh else had to say um exam you

01:26 to get three, I'm sorry, is uh it's kind of touches on

01:34 of genetics. It's not it's not lengthy unit. So uh so the

01:41 turnaround comes not super quick, but it's not a whole ton of material

01:47 as there is in one and So anyway, um so let's um

01:54 with a question. This will wrap kind of what we talked about last

01:59 . So basically I was gonna show at the end of class Tuesday,

02:04 I decided I'll just wait. So the question. This can be a

02:09 on that subject and we'll I'll tie up here with summarize it here with

02:19 couple um graphs or figures. So just look at this now. So

02:24 is about the Hi no, I'm set it excuse me, bacterial species

02:32 can grow when supplied the energy source carbon source consisting of H 20.

02:38 C. 02 respectively. And nitrate a kernel except er what applies uh

03:22 , mm hmm. Mhm. Looks like we've got our we stopped

03:39 2 45 and then we just went . So let me go. I

03:44 to it's beautiful. So if you're sure, but you know, at

04:08 two of these are right, well you know what the answer is?

04:15 , Okay. I predict 100% on . Almost, almost. Okay.

04:30 it is all these things. All . So, we know hopefully by

04:36 that if its carbon source is that that's autotrophs. Okay. Um I'm

04:47 that if you know this is a except er right then it's gonna be

04:53 to anaerobic respiration. Okay, And that can be a little

05:01 You can call it both. Um but we make the distinction.

05:07 we just know that hydrogen trophy as thing can also occur in Hedda

05:15 Right, So just kind of remembering too. Okay, not all,

05:18 some Okay, um Alright, so just real quick kind of summarizing last

05:29 . So again, this should be favorite diagram by now. You should

05:34 able to do this in your sleep scratch. Right? Um the and

05:41 terms we went through last time. we kind of focused on anaerobic

05:45 And then little trophy, hydrogen trophy . So again for the zillionth

05:52 Right. So we're looking at so to be able to differentiate get straight

05:58 your head, you know, are talking about electron source or the except

06:03 right. That's going to determine what of respiration is going on. So

06:07 look here, what's the terminal accepted the oxygen or something else? Um

06:12 source. Right. Is it organic in other words, complex, organic

06:18 , protein fat. Right. That are the sources for hedda troughs.

06:24 same thing as chemo organa trophy. I've heard the term chemo head a

06:32 . But uh are you a little ? Right. That's gonna be inorganic

06:36 here. And so we looked kind last time at um so here are

06:42 continuum, right. For both nitrogen , sulfur compounds which are commonly those

06:47 of those are used both by the . Other types are used as terminal

06:52 ear's. And so it's kind of you can compare them across the

06:57 And so the more basically the point with both these sulfur natural compounds is

07:04 more reduced forms are the ones used a source. It becomes oxidized and

07:09 supply the electrons. So your ammonium , uh hydrogen sulfide, elemental

07:16 These and the more reduced forms that oxidized. Then the more oxidized forms

07:22 reduced and these are these are the for a terminal except er Right.

07:28 so bacterial types Archaea, Archaea types use different combinations of these things.

07:33 can have little tropes that use that ammonium and they can re spire using

07:39 . You can have different combinations. depends on the species. Um And

07:45 these terms similar Torrey dissimilar Torrey. , similar Torrey process is one in

07:53 it leads to the organism that's doing . It holds on to that

07:58 right? It assimilates it into its . Okay. The opposite is dissimilar

08:03 . The process leads to the loss the molecule from the south and obviously

08:10 that case it's free for others to it's not holding onto it.

08:14 Um Mathon genesis So things remember about . It's strictly it's strictly a process

08:24 only in a certain group of Okay, so um called obviously

08:31 Okay. And so you know it a process that uses hydrogen. So

08:37 kind of um is it hydrogen a technically? Yes. Okay because it's

08:43 hydrogen. They're oxidizing it um but for production of methane. Okay.

08:48 reduce CO two. So you know put the term a town genesis on

08:53 because it refers to that specific We know that those that do this

08:57 this keel group. Um Anyway um questions about this, the last bit

09:04 got to do today is ah photo . Okay. And your familiarity?

09:10 sure. Well, I know because took intro bio is uh oxygen IQ

09:18 the way plants do it. That's what that's your introduction photosynthesis. So

09:24 note that. Um and that's what see. My goodness Sorry about

09:32 Um mute goodness. Okay, stop . Alright, so um let me

09:47 so um so you're familiar with this this process here? Right. Has

09:52 two photo systems will go through Um So cyanobacteria remember they ancestors of

10:02 way back. Right? They're the who introduced the oxygen in the atmosphere

10:05 through this oxygen in photosynthesis. And um has two photo systems um the

10:14 water is the source of electrons So again, in photosynthesis we are

10:20 talking about redox reactions, the oxidation molecules the electrons being fed into the

10:27 . So it's all still has that concept here. Okay, um it's

10:32 that light obviously is kind of the force behind this. Right? And

10:37 um uh so again, I know just you've seen this, you

10:43 you've seen this scheme before. Um when we look at bacterial types that

10:48 than cyanobacteria that there are other types photo trophy. Beyond this.

10:56 but um you can kind of easily them because in in this oxygen in

11:04 we call because the process releases Um the other types have either one

11:12 two. Right? So we have photo systems in the in in that

11:16 this form appear that the plants, and cyanobacteria do. But then the

11:19 types have either just one or to both. That's how we can differentiate

11:26 . Right? So they have either the system to Okay. And we'll

11:32 at kind of the how that operates that's an easy way to distinguish these

11:37 bacterial types of photosynthesize. They either one or two. They don't have

11:40 systems. Okay. And there's another , What are these guys?

11:46 Um Very different. So you can to the ones in the green

11:51 Okay. What I call chlorophyll based , chlorophyll based photo trophy. The

11:58 in the red box is completely different that doesn't involve chlorophyll at all.

12:03 thought to be a thought speculated to maybe the first photosynthetic system that evolved

12:12 earth. Um It uh and again not a chlorophyll based type of system

12:19 does of course involve like absorption Okay so that that's actually what we're

12:24 start with first is the one in red box there but first let's uh

12:29 when you see the picture before we . So these pictures here, these

12:33 all types of santa bacteria. Santa bacteria. Very diverse group.

12:41 can see all the different morphology. there single cells to clusters of different

12:47 to like different arrangements of cells but very important uh in the context of

12:55 life on this earth. I'm sure all know the importance of photosynthesis.

13:02 . And so let's take a look this question and I'll keep rattling

13:06 Um So you know, so we about this back in chapter one.

13:13 think the example of a basic example the ecosystem, right? You have

13:21 consumers producers. I'm sorry, bottom level consumers, different level of

13:27 . Right? And so the foundation any ecosystem where you're generally you're you're

13:33 types that are like your cyanobacteria, and plants. Okay. But certainly

13:39 other photo tropes have their contributions as . But obviously, you know the

13:46 of the oxygen IQ types are very . Alright. So here looking to

13:54 So photo trophy in general, whether of whatever type of photo trophy there

14:00 gonna be things in common among all groups, but not everything necessarily.

14:20 . Oh sorry, keep answering. haven't stopped yet. I can see

14:27 grass grow. Okay, I'm going hit this. Okay, so you

14:34 let that influence your decision what you saw or not? I counting down

14:58 754. Alright. We got the the camel with two humps there.

15:14 . Uh B uh um who answered As in boy. So so so

15:29 people have their hands up, always the questions and who else answer

15:34 You're all censored A shy one down like this. So why is it

15:44 . You can sit back I'm asking because you had your hand up,

15:51 said you answered B. That's a answer. Use this that's your

16:05 Don't use the bag test we mentioned time. Right. It's okay.

16:11 . Can anybody give you a different ? Yes, back there.

16:20 Right. Photo Metros, don't you ? Oh to correct. See that's

16:23 you're gonna say. Right, Okay. I should have drawn it

16:30 . Okay. Alright. Yeah. it's fixing sio two is not necessarily

16:36 although it's common. It's not going be applicable to all photo troughs.

16:43 ? Because you have photos to Alright, so hetero trophy is the

16:47 term there. Okay. Um so uh some of the stuff I

16:55 you already know, it's just gonna more of a rehash. So um

17:00 kind of photo trophy is going to based on having a light absorbing

17:05 Obviously some sort of some type we're to absorb light. We're gonna capture

17:12 energy in terms of photons of Right? Um The membrane is always

17:19 right? In these kind of processes , right? Membrane allows you to

17:25 a a matrix to stuff it full of these photosynthetic type pigments. Um

17:36 as well as providing a because we're be producing a proton gradient. So

17:43 need that membrane kind of to differentiate put protons on one side versus the

17:48 . So um the and then of converting that light into some type of

17:54 energy. Right. Okay. So are things that are gonna be common

18:00 what may not be our photo analysis . Okay, so these are the

18:06 that supply electrons to the process. . Very common. But again,

18:12 to all photo trophy. Okay. first example we look at is one

18:17 doesn't rely on photo analysis. so again it's the same like with

18:24 you have an electron source, you eternal except er you have the same

18:31 operating in a photo trophy as Okay. And so uh we can

18:39 based on what I mentioned earlier chlorophyll versus non chlorophyll based. And we'll

18:45 with this one first. Okay. so and that's the bacteria adoption.

18:51 so if the adoption name rings a uh if you recall the inner workings

19:00 the eye and rods and cones, , adoption is in there how you

19:08 light proves that into a visual. there is some uh similarities with that

19:19 that same adoption. And so it this system um doesn't rely on a

19:29 much in redox. Okay. In words there's no there's not electrons feeling

19:34 system right? It's just really absorption light energy photons of light.

19:40 And so it's done with this protein adoption or material adoption molecule. So

19:47 archaea or halo files or salt loving they were first found in this group

19:53 of archaea. But since this property kind of been transferred to other bacterial

20:01 beyond two bacterial types beyond archaea. . And this has to do with

20:06 we'll talk about in the next unit gene transfer, right passage of genetic

20:13 between cells. Okay. Um and that mechanism is thought to be responsible

20:19 just for how this feature spread from to other bacteria. Uh but it's

20:28 a system that the light absorption is out by this retinal combination of protein

20:39 um this right now right now is light absorbing molecule. So you see

20:45 here and it's bound conveniently linked to protein. Okay, shown in

20:54 Okay, so so it's basically the and retinal combined together. Okay.

21:01 the retinal is the light absorbing Alright. And so when light is

21:07 by that right now there's a there's um configuration change around that base.

21:18 , so it goes from assist to trans shape. Okay. And you

21:25 the switch right here. Okay. absorption causes that. Okay. And

21:32 because it's bound to the protein, kind of change in direct now causes

21:37 change in the protein it's connected Okay. And that change results in

21:43 pumping of protons. Okay, so have protons being pumped out as that

21:50 absorption occurs. Okay then. Not here, but there will be a

21:57 P synthesis. Okay, that will couple of proton transfer to a teepee

22:07 . Right? The protons will then back down. Okay, so there

22:12 uh an A. T. Sentence that is part of the process

22:15 course. Right, so it's basically light driven proton pump. Okay.

22:20 the energy then from that proton gradients through going to an A.

22:25 P. S. The same Same mechanism we saw before.

22:30 Um the canucks emotions mechanism. So now these things appear purple in

22:39 Right, so remember like you uh an image you see the color of

22:48 is due to the like certain light being absorbed and the other is being

22:55 . Right? So with this it green light. Okay. And hence

23:02 kind of purplish color. Okay, areas that are full with these bacterial

23:07 and these are generally aquatic bacteria. waters will basically be purplish if there's

23:14 high density growth in that water. things like the red seas has high

23:19 concentrations and you'll see purple pools purplish within that, see that represent the

23:27 of these organisms. Okay. Um they often call these the membranes where

23:34 are densely packed with these um reduction purple patches because they reflect that

23:40 Okay, so um and so one of the main things is there

23:47 no foot tosses. So it's just once they absorb light and the proton

23:54 pumped out then they kind of revert to the original cIS form, then

23:59 hits it again and it repeats the process. So it just kind of

24:02 over and over again. So there's there's no like redox going on

24:07 it's just light absorption and the proton out. Okay. Um so so

24:13 fatalities. And these are hetero So these are hetero tropes that are

24:17 this, right? So they have way of getting energy um just to

24:22 their hetero traffic lifestyle. Okay, um any questions about that?

24:30 so alright, so a non chlorophyll , this is not a chlorophyll type

24:36 at all. Okay, Alright, chlorophyll based, so this is going

24:41 be what you're most familiar with the scheme, you've probably heard it called

24:47 . Um So obviously this is this of photo trophy is probably the most

24:52 . Okay, on earth. It's you think of all the areas on

24:57 uh in terms of vegetative growth plants at the water and marine environments,

25:05 and bacteria represents a lot of activity . And so um the santa bacteria

25:14 bacterial members and so as collectively this we call them oxygen it.

25:19 because the process uses water. We water an auction to buy product.

25:26 , so again because we're involving membranes , we're gonna stuff these light absorbing

25:33 into a membrane, it's gonna have features to do that. Right?

25:38 so um chloroform molecule and its non tails if you will hear that gets

25:47 in the membrane. The chroma four the light absorbing portion of the

25:51 Okay, so we have a metal in their manganese in chlorophyll. And

25:56 this is the the molecule in plants in santa bacteria. Remember santa

26:02 are the forerunners of of the chloroplasts algae and plants. And so they're

26:09 going to share that same feature. And so the chlorophylls themselves are combined

26:17 proteins and other we call accessory pigments kind of enhance expand the the wavelengths

26:25 light that can be absorbed. Um absorb blues and reds. These accessory

26:30 come in to contribute kind of maybe more of the orangy colors. Um

26:38 he arranged these in little satellite dishes there called antenna complexes. Okay.

26:44 so a combination of proteins and horror and these accessory pigments. Okay and

26:52 , high densities are stuffed into the . Um Remember that cyanobacteria will not

26:59 organelles, right? But it will its membrane and just highly folded,

27:05 up stuff that fold these pigment molecules actually have little extensions between them like

27:13 . But again, it's all one of continuous unit that's kind of folded

27:18 . Okay, stuffed full of these molecules. And so um so light

27:24 occurs. Right? So we have and it'll bounce around being absorbed by

27:31 chlorophylls that are kind of in the orientation to absorb it. Okay.

27:36 then we'll kind of shuttle that energy the middle. It's called the reaction

27:42 . Okay. And this is where redox then occurs. So you have

27:47 light energy uh pushes those electrons to higher energy state and out of the

27:55 to um electron carriers that will be out here. Okay. And then

28:03 will be handed off to different electron members of electron transport chain in that

28:10 . Okay. And so uh so course light absorption is what triggers

28:14 Okay, so if electrons are being out, Okay, this has essentially

28:23 oxidized. The core fill or photo is what we call these has become

28:30 because light has driven electrons out of . Okay. And so it then

28:36 to become reduced. So grabs on water right? To replace those

28:44 Okay, so water will be here part of that analysis reaction.

28:50 And it gets oxidized. And so why auctions by product. So um

29:01 we look at core fields, here's a wavelength spectrum for that.

29:07 then we look below at bacteria. fields, folklore fields, we're gonna

29:12 in santa, bacteria. Other bacterial will have what's called bacterial chlorophyll similar

29:19 does absorb longer wavelengths of light than chlorophyll. Okay. And so you

29:26 see kind of it's outside the spectrum course we're particularly out here. That's

29:35 low energy infrared range. Especially out , right at that almost 900 nanometer

29:47 . That's now we're getting to the . R. Which is very low

29:50 . Okay. And that low energy to what it can use as a

29:58 donor. Okay. So the core core a film system um has more

30:08 associated with it. So it's able then strip electrons from water.

30:13 The systems of bacterial chlorophyll. Not much. So they don't have enough

30:18 to really pull electrons from water. why different donors are used in those

30:23 . Right? Which is why it's it's called an oxygen IQ. Or

30:28 because they don't generate 02. Okay they use if you're using something other

30:33 water you're not gonna form oxygen. ? So they use things like

30:36 Two S. Iron etcetera. So um and so in in a

30:44 know in an aquatic system for example would have your santa bacteria, your

30:50 gonna be at the upper layers near surface. Okay? And then as

30:57 absorb the light they absorb the other then trickles down. Okay so this

31:04 light over here. Okay then trickles and then they're they're gonna occupy the

31:10 layers in this example in this water . And so and actually tend to

31:15 of be somebody's tend to kind of just in the sediments below.

31:20 And so um but they absorb lower light. Uh and of course you

31:27 still use it and produce energy that . Okay. Um and so the

31:34 . Scheme looks like this. So um we have two photo

31:42 Okay. So and the order is 21 simply because that's how they were

31:49 in that order. Okay. And the light reaction um light absorption by

31:56 system. Two knock electrons out. . But then for telesis is what

32:03 electrons to feed. So we're feeding . So we're going from water for

32:07 to photo system one to N. . D. P. Okay.

32:13 that's kind of the flow. And so of course it's oxygen

32:18 Because we're forming oxygen. Okay, a byproduct. So um and so

32:25 PS two is associated with a teepee . Okay, so we by the

32:30 mechanism we're going to pump protons But it's of course it's driven by

32:35 . Right? So it's photo phosphor but mechanistic lee is the keamy osmosis

32:42 . Right? So protons pumping and P synthesis. We generate a

32:47 P. S. Okay. Now initially high energy here and then progressively

32:55 energy as they go downhill being And then two PS one.

33:02 so then PS one absorbs like again electrons and they come back down ultimately

33:08 a D. P. Okay, kind of a general rule. And

33:15 applies to any living thing, is you typically see N. A.

33:21 . P. In A. P. H. In processes processes

33:26 are anabolic anabolic processes tend to use A D. And A D.

33:31 . And A D. P. . Right. Um Generally not always

33:36 case but generally that's the case. so these are gonna be used for

33:39 two fixation right? Um now the so that's the flow of electrons and

33:47 again the energy is all used to CO two because these are of course

33:51 . Photo water. Right? Um flow electronic you see there right in

33:58 D. P. And the ndp to any D. P.

34:00 Okay. Um so now the variations here for one last thing. So

34:09 is kind of a schematic. You need to you don't need to know

34:13 pasto sand. And you know, just showing a diagram here.

34:17 That is just kind of redox right? We have cider, chrome's

34:22 we saw in respiration, quinones. we saw respirations. These are common

34:27 used in these kind of electron transport . Okay. Not obviously like chemically

34:33 but similar in terms of how they . Okay. And so now the

34:38 to again associated with a T. . Production. Okay, proton

34:44 And then the A. T. . Sent this. Okay. And

34:48 photo system one comes in and associated an A. D. P.

34:54 . Formation. So energy production using and again, C. 02 fixation

35:03 a lot of energy. And so this is worth coming from. Okay

35:11 the the next scheme. So before go, any questions about this?

35:29 mm. Yeah. Right. Mhm correct correct. I'm sorry question.

35:49 um yeah a teepee formation. So we go into these these other bacterial

35:55 that have the bacterial chlorophyll they're gonna have just one of these two

36:00 not both together. And so um don't see that here. The first

36:08 is this anaerobic photo system one? . Um so we called the green

36:14 bacteria. Okay they have so the one is associated with N.

36:20 D. P. H. Okay um they use things like hydrogen

36:26 , age two uh something other than . Okay the so they do have

36:33 reaction um They also certain species can these Klores OEMs. Okay so these

36:40 organelles in the sense of a lipid layer. They do have some some

36:46 in there but they also have lots protein in there as well. Um

36:50 basically stuffed full of the bacterial Okay and uh many of these species

36:58 they collectively forming what are called chlor . Okay basically light absorbing conclusions,

37:05 whatever you wanna call it. Um so now you may think okay well

37:12 don't have the PS two. So do they make a teepee? They

37:16 do they just don't have it associated a PS two system. Okay so

37:21 you can see that even from the in the example of H two

37:26 you're still forming H protons as a , you can still form a proton

37:33 and there will be an 80 ph with it. So it can still

37:37 this. It's just not specifically linked a PS two system. Okay.

37:41 they certainly have the capability of making Tps this way. Okay. Um

37:47 and again they are autotrophs. So gonna use the energy to fix

37:51 02. Okay, so the other here is the one that has PS

38:02 but no PS one. Okay. these types called purple non sulfur bacteria

38:11 actually have a they don't have a to the system. There's really not

38:16 photosynthesis reaction here. Okay. These absorb super low energy light, basically

38:25 region. And that energy is so , it's not capable really of being

38:32 to extract electrons from a source in same way as in the photo analysis

38:40 . So basically it cycles the So it's what we call cyclic photo

38:45 relations. So you see the light uh and here's the P 8 70

38:51 see here that refers to the wavelength light. So it's very low.

38:55 ? 100 70 nanometers. And so that is enough to excite the electrons

39:03 be knocked out and then passed But not enough energy that it can

39:08 electrons from something else. And then feeding the process. So it basically

39:13 cycles these electrons around and keep reusing . Okay, so cyclic photo false

39:21 . But you're still generating a proton . Okay. And using that to

39:27 form A T. P. So it has the PS two system to

39:31 able to do that. It just to cycle electrons sort of using photosynthesis

39:36 . Okay. And so the uh when we look at actually this group

39:44 general, um they're a trophy. they don't really fix so they don't

39:50 C. 02. Okay, so use the A. T.

39:53 S of course to fuel their head trophic metabolism basically. But they're actually

39:58 very diverse group that they're capable of metabolisms. Right. Using light to

40:05 this uh eating organic materials like ahead trophy wife. But can even it's

40:11 seen they can even be a little as well. So they kind of

40:14 span multiple metabolism. So very Okay, but for our purposes we're

40:19 at photo trophy. So uh just remember these couple of things about the

40:25 photo foster relation. Their photo header . Okay. Kind of the main

40:30 here. Okay. And of course associated with Photo system two.

40:35 so we'll put this all together here this summary. So here is three

40:41 . Okay, so oxygen in plants, algae, cyanobacteria.

40:47 Two photo systems this reaction. And they're on a trophy.

40:53 so we look at the those that the bacterial core. Okay. The

41:01 group we looked at green sulfur bacteria one only. Right. So they

41:05 form the DPH with that. But mentioned they do have a way to

41:10 a proton gradient as well and get . But it's just associated with the

41:15 two system. They use these things donors to their system. Um Which

41:22 they don't produce auction. So it's an oxygen IQ process. Okay.

41:26 And they are autotrophs as well. the last group we just talked about

41:32 purple non sulfur. Right? So cyclic to foster relations their photos to

41:39 they are not autotrophs. So that away and but they have the PS

41:44 system which is the proton pump and driven pump and a teepee formation.

41:51 so those are three systems. Um questions about that. Okay so let's

42:01 at a question here. Okay so about that. If you have any

42:24 , feel free to spit them out if you like. Um So remember

43:02 next week we start unit three. so this essentially ends unit two.

43:18 . Okay count down from nine All right. Here we go all

43:41 the map. Gotta do some Okay let's see Kia those are the

43:52 adoption. So it's not it's not . Is false cyanobacteria are oxygen

43:58 Not an oxygen IQ. Uh purple sulfur photo possessing photo system one.

44:06 two. We just saw that. A B and C. A.

44:10 D bacterial adoption based photo trophy photo . That's true. So D.

44:17 in dog, is true. that's it, folks. We're

44:23 enjoy the weather. And of you're gonna go now study, for

44:30 , to

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