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00:00 Okay, so there we go. , so any other questions? So

00:07 four. So here um your book go into a lot of eukaryotic cell

00:16 stuff. I'm not Okay, but will I will show a picture of

00:20 eukaryotic cell just for comparative purposes. . Our focus is on precarious cell

00:28 etcetera. That's what uh focus Okay, so as you read the

00:34 , you know, stick always stick turned out that exam review sheet,

00:40 example, already available. You're reading book, just kind of don't read

00:47 that's not gonna be covered. so I'm not gonna ask you about

00:51 carry out of cell structures. I'm gonna focus on carrying cell structure.

00:57 , so uh material where many different morphology right here live in a

01:09 A number of these types. Um basically the single cells themselves can have

01:19 number of different arrangements. Uh So basic rod shaped, the cell i

01:24 shaped proxy uh comet shaped called vibrio these guys. Okay, um

01:34 Um Of course spiral is shaped uh . Alright, this is kind of

01:38 unusual one. You can see the types. You know whether it's a

01:46 chains, extracted caucus or uh uh like staff or even rod shaped.

01:57 singular and chains or the they're all even they're all uniform rather all

02:06 They're all rock and they're all uniform . The metamorphic is not. That's

02:13 kind of characterize is not uniform And have these branching forms um the

02:22 shape to them. So they're not shape so that we can use the

02:28 to describe those types that don't have uniform shape. We don't call them

02:33 proxy or bacillus or what have Okay. More for mankind is weird

02:38 non uniform shapes to them. So um All right, so here

02:45 part 11 is kind of what kind takes the approach of, what's the

02:52 on the outside of the cell And go continue to go inward.

02:56 So we're going to start with more the peripheral stuff. Right? And

03:00 part two is kind of more about inside the cell. Okay so that's

03:04 of the approach here. So here's question. So if you join the

03:12 uh liquor points, I've switched points capital. Next Tuesday because the book

03:18 is a shortage backlog codes or whatever so they'll be in today tomorrow so

03:26 just push it back until Tuesday. do check upload these to blackboard.

03:33 keep checking them and you're seeing your . Okay So you should be able

03:42 all these things today. Um So going to let you answer then I'm

03:52 take a snapshot of the results. gonna see this slide again then we'll

03:56 over it. You do cold turkey . Alright let's see let's do a

04:09 down here. 10 9 seven six 43 2 1. Alright. Here

04:26 go. Beautiful. Okay. Alright onward we go. Yeah

04:46 Okay. Alright um Next let's look the comparison between the two.

04:53 Not not to scale exactly here, . It's gonna be smaller but but

05:01 for only for competitive purposes. So what's obvious is eukaryotic cell.

05:06 the left. Lots of members. holdings, Right. Um compartments um

05:16 nucleus containing chromosomes um uh Right You can't see stuff in there but

05:26 don't see the degree of membrane folds these kind of things. And there's

05:30 such thing as organelles, improved Um one chromosome so relatively simple compared

05:36 a new stereo. Um But of they they have certain things in

05:41 Obviously they have DNA and proteins They have a cell membrane. Of

05:46 . Right. That defines anything. But the is gonna have some some

05:51 to pro carrying dozens and and vice . Okay. And so that's gonna

05:55 Our focus is gonna be Over So remember your hopes the size

06:02 Right. 1 to 10 Microns. micron. These are generally greater than

06:09 microns. Okay. And up. , so size differences. And of

06:15 uh and I have all this here well. Let's just look at that

06:19 . Okay, let me move this the way. Okay, so you

06:24 out uh you see that chromosomes you like 46 of these in yourself.

06:30 . A defined nucleus as you can their membrane bound nucleus containing chromosomes.

06:39 lots of different organ alum's central. sexual reproduction and a sexual reproduction can

06:46 with uh So all plants have sold these are gonna be so he looks

06:53 different from bacterial our so nuclear nuclear . It's not obvious. Right?

07:03 oid basically means its nucleus like it's not it's all it is.

07:09 just the area occupied in the cytoplasm the chromium. Okay, that's

07:15 So you can give it it's kind grainy appearance. Right? And you

07:19 see the nuclear Lloyd here is kind right here. Okay. Is

07:25 So it's simply just a site of area that that's it. Okay.

07:30 more. There's no membrane around Okay. Yeah. And you can

07:36 clearly from these two pictures, the of stuff that's a eukaryotic cell and

07:42 size difference. Right. So precarious course have like organelles, site of

07:49 . Right. Remember those were a series of different types of filaments,

07:55 filaments that have different functions in you ons. Right. Everything from an

07:59 spindle during mitosis or mitosis to micro . Right. For. But you

08:08 out uh what are called intermediate filaments anchor organize together different many different very

08:15 organized complex side of skeleton. Not not so in bacteria. Um

08:22 so well again is very different material compared to plant cells. Of course

08:27 fusion is our motive replication again. taxonomy. Right, bacteria, archaea

08:33 carriers. Okay, so uh let's at this question. So you're looking

08:40 this uh I want to show you over to you the appropriate cell and

08:47 show you all the structures and then take it one by one after

08:53 Okay, so let me open the , sorry. Okay and that

09:01 Okay, so the most abundant molecule the bacterial cell really in any cell

09:06 be honest. So I think in of quantity of modular cell quantity

09:28 All right, let me do 10 2nd countdown here is here and

09:37 is. Okay, Clark is going speed that up a little bit.

09:47 seconds one. Okay, we'll go . Okay, so molecule um 3rd

10:11 . As a cat. So he see because was the proportion of on

10:18 percentage basis? Yeah, Here is water. So water molecules are gonna

10:33 the most abundant in terms of molecules cell in any little thing.

10:37 Life is 70% water. Give or . Okay, so you're gonna have

10:44 ? Okay, second would be Right? Protein numbers. In the

10:51 of the thousands. Okay. In south numbers and millions more billions

11:00 Um How many how many um on quantity basis? How how many how

11:08 D N. A present training? one be granted. Okay, there's

11:21 one of those. Okay um this get the water thing, anybody challenge

11:31 on that one. I'm okay with . All right, so,

11:37 so let's look at the structures I told you. And so one of

11:42 things to um mentioned first is this cell envelope. Okay, um I

11:53 it because to say because the number is is so every cell is defined

12:03 this, just like this, By that, right? You know

12:06 ? So, you know, you're to sell you see, see this

12:10 of the microscope that has this boundary it. Right? That's what makes

12:13 cell cycle classic memory. Okay, , what for bacterial pro carry it

12:22 . Alright, I throw archaea in as well, of course, is

12:28 out here. Okay, what's in boundary external to? That's that's the

12:38 to go negative and positive. Something . You have a lot of different

12:46 out there, depending on the type bacteria are th Okay, so,

12:52 why I use the term cell envelope describe what that is. Okay,

12:58 , um All right, so, the membrane is gonna be a medical

13:05 them in a violator kind of Okay. But of course, with

13:10 have proteins to because proteins help things out of the cell. Right?

13:14 , they can have a catalytic activity Okay, gradients, we'll talk about

13:20 . It's very important in terms of be able to transport and energy things

13:26 sell later uh in the cytoplasm, course the cytoplasm, the cytoplasm and

13:33 all the cytoplasm is basically everything that's that boundary within that the silos all

13:43 the angriest portion. Okay. Of cytoplasm. Okay, so the sight

13:50 this all of course contains water, the different various chemicals, ions,

13:56 . Okay, um nuclear. We talked about that. Right? The

14:01 occupied by the chromosome rhizomes are visible electron microscopy into a bunch of

14:09 Decided to sell probably the number one any cell on this planet is to

14:15 proteins that are needed all the time everything. And of course if you

14:20 protein to be made there are lots items. Okay, so you're gonna

14:23 lots of resumes. So so here see the appearance of Okay, what's

14:32 ? That's right. The envelope. I just try one more time

14:36 So now you see it now. , So it can be a cell

14:40 , right? That can be part the envelope now. And uh for

14:45 gram and gram positive, gram gram positive and negative distinguished by the

14:53 of that cell wall and cell different sugar matrix, different sugars than

15:01 sailors is. Okay, sales we implants all so very different uh and

15:08 the thickness of varies from gram You see they're gram negative has thinner

15:17 also also has an extra layer. you actually see three layers here.

15:24 , in the gram negative gram positive the here we use that because If

15:34 go back just one in here. here's a cytoplasmic membrane and because bacteria

15:40 archaea have stuff generally have stuff on outside refer to that. It's the

15:50 that bounds everything and even stuff So so wall is outside that inter

15:59 . Okay against robert let me think has an extra third layer an extra

16:06 memory. So I have that sandwich structure remembering so we'll remember it.

16:14 . And then in the cell walls thin in the the uh plasma.

16:20 plasmids we see here are small pieces D. N. A circular piece

16:28 D. N. A. They A few genes. Okay, their

16:36 is generally 10 times less than that . Um These are the pieces that

16:44 transferred from cell to cell. So this can be transferred to another

16:50 cell. Okay. Not all pastors do this but something we'll talk about

16:56 uh here but it's you know antibiotic are just only requires a couple of

17:04 that we often find those classrooms that be transferred himself himself. That's how

17:09 can spread positions um now and you have one or more of these and

17:20 sell but it may have not. just depends. Okay so external

17:25 things like. Okay so both of things are made of the same

17:35 Okay, they do differ in terms how they function. So pillai are

17:42 more numerous and short. Okay I'm you got that backwards, erase that

17:53 informants Pillai are longer typically and a for sale the pill. I typically

18:02 to have more specialist functions like a pilots. That's how they transfer plants

18:08 cells. Other types of pilar involved uptake of certain molecules involved motions.

18:17 talk about that. So there was difference between those two in terms of

18:21 the function particularly about attachment. So forming bacteria, those that knew that

18:29 have it's essential to their ability to a biofilm can stick to stuff.

18:36 motility. There is a type of that we'll see with life but kind

18:43 the traditional type of utility you're used seeing they have that two different than

18:48 you carry it does it. But for motility and then as you look

18:55 you see a purple structure appear. , that's for those that have

19:01 It's a capsule. Okay. Be thick. So a capsule basically is

19:08 back up back up once, basically the structures that are external to that

19:17 memory. Right. And so in so you often see this associated with

19:22 pathogens. Right? The the pneumonia the the meningitis organisms very thick

19:30 . Okay, so by covering their structures, they're really kind of hiding

19:37 from the body because remember the body for these antigens to mount an immune

19:42 to it and those things are being up and then you can't see

19:46 You can't see it. Right, capsule is kind of a way to

19:50 itself from the body. Also a capsule. Makes it hard to sensitize

19:56 ability to get rid of pathogens. of many functions is certain cells you

20:01 that favors a path and eat them , take them and eat them.

20:05 , capsule makes that less easy to . Okay, so that's why you

20:10 it as a as a balance Okay, so in terms of what's

20:18 this page, yeah, can be factors for certain types. So kind

20:24 jell o um capsule for sure can a factor. So uh toxins can

20:35 religious factors. Uh Other things we'll about those as we go along.

20:41 um so yeah, it's kind of of the structures will get more specific

20:47 certain of these. Okay, and you kind of begin with again,

20:54 on what's outermost and then take it , go further in the cell.

20:59 , so black. Okay Alex is of a generic term for three different

21:07 . So typically it's a sugary can somewhat protein matrix Ok, surrounding the

21:15 . Okay, almost all cells uh all bacterial cells or cells produce some

21:25 of news if you will. Some than others. Just as a byproduct

21:30 kind of a and you notice there certain facts are really good at this

21:34 you have a Petri dish and the contains like saying excessive sugar that will

21:41 on that plate. Like a they they do so much this extra material

21:50 just loses out very noticeable. So but there's 22 ways to do

21:58 Okay. One is what you call capsule that we just mentioned.

22:05 capsule is very tight. See this a capsule right here. Very tight

22:11 the cell here. Very tight Uh Right, you can have uh

22:26 else capsule in itself. So it's genie could affect a slime layer is

22:34 it's a loose a sandwich as you here. Right? That's the slime

22:39 and that's the capsule. Okay. capsule stain, you can see here

22:46 halo, right that clear area around pink cells that the capsule.

22:53 so just kind of byproduct this stuff made. The cell doesn't use that

23:00 of secretes it and it kind of hangs around the outside of the

23:04 Okay, now uh a random thing , but you know those that produce

23:11 slime layers, it can help them this stuff. Of course, it

23:15 be some bit of protection. But it's it's not like it's a

23:21 that that's producing this contrast to a . Okay, biofilm of course is

23:32 completely different because it's a product of cells come together uh of the biofilm

23:42 species. Uh then they uh code materials that make is basically the

23:51 Sugary matrix. They produce, it's of the glue that holds together you

23:58 that. It's really the essence of biofilm. Okay, so um so

24:05 things that are produced external to the some of these kind of buying

24:10 There's very capsule. Okay. Um questions about that? Yeah.

24:25 A capsule is because the slime layer kind of more random. Right?

24:29 not the amount of slime produced by , made very substantially what's growing

24:36 And it may prove a little or lot. It's kind of like kind

24:39 a random thing kind of based on than what's what it's eating. The

24:44 is a very specific thing. So gene coded all sales will have it

24:48 capsule. They'll produce it. So , very very different from no

24:55 Yeah. This might be a little of an odd question. Can that

24:59 matrix be processed into? I'm sure probably work on that, but I

25:09 think it would be that difficult to a few things. Uh I'm just

25:14 sure. As with all those it's if you're going to develop that

25:19 comes down to people buy it will money. Okay. And trust

25:25 I want to buy a tech. I've been involved, those kind of

25:28 and it essentially comes out that it something that medical importance maybe then we

25:34 a better chance to but yeah, comes down to Yes, I would

25:38 it can be done with the utility uh for um Yeah. Good

25:46 And then you. Alright. Um . So I'm assuming there's a familiarity

25:56 eukaryotic movement. Okay. Is so Well again and the movement the movement

26:07 a whip with the. Okay. like this. Right? A bacterial

26:15 propeller spins spins. That's the movement creates is a rotary movement.

26:23 So this is showing a this will a gram negative because we have an

26:29 and inner membrane. So grandpa can multi as well. So but regardless

26:34 have a structure that is a based that anchors the um and uh then

26:46 called the basal body. Of So that kind of was what anchors

26:49 the membrane and then you have this part they call the hook.

26:54 That rotates. Okay. And that's kind of motion we're producing here.

27:00 , so you're gonna see the terms okay, uh protein units called um

27:16 have uh the protein content can differ from strain the strain. And it's

27:29 structure that can elicit the response. . And so many decades ago scientists

27:41 particularly with E. Coli and Okay. Cause food poisoning their their

27:49 path in times of both with that . They worked out this ancient.

27:59 you're probably familiar with um the black the chipotle E. Coli.

28:05 Which is what's called E coli Okay, so this one is referencing

28:11 oh that's the engine. That's part the grand negative structure. Right?

28:16 you can also identify it based on H. Number two. Okay so

28:21 number it's basically a particular type of which contains antibodies to that specific

28:29 Okay so we worked out no need have these antibodies to these various different

28:36 and to identify. So there is food borne outbreak of E coli we

28:42 identify which one it is. We just do the simple serological test.

28:47 know the antibodies to it if it up with 157. Boom you go

28:52 that's that's the bad several but you identify them by these types of

29:00 And H. Okay so the and you can identify are used as a

29:13 pool. Uh the nature of the morphology or arrangement better work.

29:21 Of of these types because they can different arrangements. So you can have

29:28 just one flagellum at one end. can have a brood of you can

29:36 all over the set. Okay. it's very terminology for these things.

29:40 don't I'm not gonna like this are for you to know that because it's

29:44 something that's very common. Use that an identification tool but nonetheless the point

29:48 is that you can have different arrangements is a species specific. You even

29:54 a type that is um one ft . This end and one on that

30:02 . Okay and only uses one at time and it can be both.

30:08 he's one this way one is that . Okay and that sort of thing

30:13 call it. Uh One thing you note is that all these things you're

30:18 that our total they're all shaped. most bacteria are motel are line shape

30:28 very very very they're actually in their vast majority of things that are

30:37 Um Okay the motion of this, motion with this type of jump.

30:42 again it's rotary. It's based on they moving clockwise or are they moving

30:51 clockwise? Okay so um so a in the run versus a tumble.

30:59 so. Okay and so it's all the proportions of those. Okay so

31:10 and what's driving this? Uh So means movement. Right so photo taxes

31:17 movement toward light which many things on have. Uh this is chemo taxes

31:24 based on the presence of chemicals. what that's what's driving the motion.

31:29 so you have a picture of Okay, I'm gonna just back

31:34 So you see On one end of cell there will be I'm exaggerating this

31:43 effect here. Right receptors At one . Okay. Little little protein receptors

31:53 one end of the cell. And so so we called chemo attraction,

32:03 based on the presence of a chemical one of the chemical chemical is going

32:07 be nutrient things amino acids, sugars they can use to grow on.

32:13 . And so if the receptor binds of these that will induce it to

32:22 out a run a straight line Okay, so it's moving around and

32:30 senses and the run takes it to chemical, more binds and equal

32:38 So presumably it's going toward more and nutrient which it wants to do.

32:44 you can use it. So that's driving the movement. Okay, so

32:51 a lesser amount of attractive then you fewer runs, more tumbling going

33:02 so you see here is a high of tumbling. Okay, and you

33:08 that because it's kind of randomly going nowhere seemingly. Okay, it goes

33:14 direction directly go this way and that this way that way. Okay,

33:18 really purpose to it. Okay, but actually going on is is trying

33:23 see if I randomly go this toy that way and this way that way

33:28 I run into a molecule because if do then that will change my motion

33:35 go from something very just random all the place to. Then something with

33:39 purpose to imagine the presence of attractant then guide it and that's when you

33:45 a a lower tumbling frequency, higher of Ron's. Okay, so what

33:54 doing is we're going from more um rotation here more Counterclockwise because that's um

34:11 is one. Okay, so you're the rotation and it's all based on

34:18 chemical down there. Getting more counterclockwise runs. Okay. And so the

34:23 is okay if I initially kind of go this way that way I'll hit

34:27 chemical that that I want and then change your emotion into going towards.

34:34 that makes sense. That's kind of . Okay. Any questions about

34:39 So tumbles to runs right more runs you've it's picked up attracting it's going

34:45 it. Okay uh so another type motion is what's called aspiring. Okay

34:57 unique motion. Uh clinically speaking, enema syphilis organism causes secretary transmitted

35:09 Um One of these types and so does have extra jello it's just not

35:16 that's like this where it's here's the and here's the flopping around doing and

35:23 I'm here general here is what's called actual filament. Okay. And it's

35:33 around the set. Okay okay so fixed at both hands cell. So

35:42 of its the passion on both ends direct around the cell when it moves

35:47 it rotates the whole thing was like corkscrew. A corkscrew. Very characteristic

35:54 . In fact corkscrew. So very to diagnose. Right? Somebody has

36:00 . You take some of the material the uh the wound the uh what

36:07 call what it is the blister and will contain live bacteria in the

36:14 And you see these great uh it's a way that motion is also how

36:24 will burrow into your tissues. Using that motion into your tissues like

36:30 very long corp And uh since this a disease that uh initially it's uh

36:37 be diagnosed fairly easily and be treated then it kind of disappears if you

36:42 treat it and after a few years may not think so I don't have

36:47 and it breaks out in a different in a rash but by that time

36:51 actually penetrated into your body and one the ways it doesn't corkscrew motion to

36:57 into your tissues. Okay. Um so again as mentioned before, you

37:07 see here the numerous appendages on are much due to right short and more

37:16 . Okay, attachment is kind of thing. Okay, attachment to

37:21 Um uh The uh Okay so for the bathroom formers they will have

37:32 It's been seen that those lacking was critical to the process. Um pill

37:42 uh as a specialized functions like transfer plasmas as you see there uh and

37:48 functions and and a type of joke kind of a weird joke completely online

37:57 . Okay this is what we call motility or gliding twitching and or gliding

38:03 . So again all that surface. so if you were in labs you

38:13 proteus miraculous on a plate. It's of a weird appearance. You see

38:17 swarming effective in that. And so can do this kind of weird

38:22 So you see the bacteria, here's pilots right here. Okay. And

38:28 it attaches, right? It will , it will get longer, then

38:34 will attach as you see here. the cell is still in this position

38:38 the pillar has elongated. Okay then cell moves forward, right? Because

38:47 pillai has deep, deep deep, you take units off making sure.

38:53 because of what connected to each it moves along, right? So

38:58 on the surface. So it's kind a very perfect jerky motion. Is

39:05 goes out. They have as the kind of a twitchy, herky jerky

39:16 check. Um Many questions. I that's a good spot. So we'll

39:24 it up with cell wall next So uh no school monday. So

39:31 weekend. Yeah. Excuse

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